Journal:

Article Title: Analysis of Chicken Mucosal Immune Response to Eimeria tenella and Eimeria maxima Infection by Quantitative Reverse Transcription-PCR

doi: 10.1128/IAI.69.4.2527-2534.2001

Figure Lengend Snippet: Localization of CD3+ cells in the jejunal mucosa. Frozen sections of control (A) and E. maxima-infected (B) chickens were labeled with a mouse anti-chicken CD3 and then stained with a fluorescein isothiocyanate-conjugated goat anti-mouse antibody. Arrows indicate IEL; arrowheads indicate parasites in the infected mucosa. Slides were slightly counterstained with Evans blue. Magnification, ×400.

Article Snippet: Seven-micrometer-thick frozen sections were incubated for 30 min with a mouse anti-CD3 antibody (clone CT-3; Southern Biotechnology, Birmingham, Ala.) diluted 1/100 in PBS containing 0.05% Tween 20.

Techniques: Infection, Labeling, Staining

Journal: Frontiers in Veterinary Science

Article Title: Heat Stress Causes Immune Abnormalities via Massive Damage to Effect Proliferation and Differentiation of Lymphocytes in Broiler Chickens

doi: 10.3389/fvets.2020.00046

Figure Lengend Snippet: Effect of HS on lymphocytes and the spleen morphology of broiler chickens. Broiler chickens were maintained in the TN or HS condition for 14 days (A,B) . Representative flow cytometry profiles of 7AAD − CD45 + leucocytes and 7AAD − CD3 + T cells in the spleen at 36 days of age. (C) Cell components of splenic CD45 + leucocytes, including CD3 + T cells, Bu1 + B cells, and other cells, and absolute number of splenic CD45 + leucocytes, Bu1 + B cells, CD3 + T cells, CD4 + helper T cells, and CD8 + killer T cells were analyzed by flow cytometry (D) . Representative hematoxylin and eosin-stained histological sections of spleen at 36 days of age. Scale bar: 200 μm. Arrows: germinal center. The data are presented as means ± SE ( n = 8/group). *** p < 0.001 compared with the TN group (unpaired two-tailed Student's t- test).

Article Snippet: The cells were stained for 30 min at 4°C with fluorescein isothiocyanate (FITC)—conjugated mouse anti-chicken CD3 (clone CT-3; 500 ng/ml; Southern Biotech, Birmingham, AL, USA), phycoerythrin (PE)-conjugated mouse anti-chicken Bu1 (clone AV20; 100 ng/ml; Southern Biotech), and allophycocyanin-conjugated mouse anti-chicken CD45 (clone LT40; 100 ng/ml; Southern Biotech).

Techniques: Flow Cytometry, Staining, Two Tailed Test

Journal: Frontiers in Veterinary Science

Article Title: Heat Stress Causes Immune Abnormalities via Massive Damage to Effect Proliferation and Differentiation of Lymphocytes in Broiler Chickens

doi: 10.3389/fvets.2020.00046

Figure Lengend Snippet: Effect of HS on the functional maturation of B cells in the bursa of Fabricius. Broiler chickens were maintained in either TN or HS condition for 14 days (A,B) . Representative flow cytometry profiles of 7AAD − CD45 + leucocytes in bursa of Fabricius at 36 days of age (C) . Cell components of bursal CD45 + leucocytes, including CD3 + T cells, Bu1 + B cells, other cells, and absolute number of bursal CD45 + leucocytes, Bu1 + B cells, and CD3 + T cells were analyzed by flow cytometry (D) . Representative hematoxylin and eosin-stained histological sections of bursa of Fabricius at 36 days of age. Scale bar: 200 μm. Dotted line: Border between cortex and medulla. The data are presented as mean ± SE ( n = 7–8/group). ** p < 0.01, *** p < 0.001 compared with the TN group (unpaired two-tailed Student's t- test). IFE, interfollicular epithelium; FAE, follicle-associated epithelium.

Article Snippet: The cells were stained for 30 min at 4°C with fluorescein isothiocyanate (FITC)—conjugated mouse anti-chicken CD3 (clone CT-3; 500 ng/ml; Southern Biotech, Birmingham, AL, USA), phycoerythrin (PE)-conjugated mouse anti-chicken Bu1 (clone AV20; 100 ng/ml; Southern Biotech), and allophycocyanin-conjugated mouse anti-chicken CD45 (clone LT40; 100 ng/ml; Southern Biotech).

Techniques: Functional Assay, Flow Cytometry, Staining, Two Tailed Test

Journal: Animals : an Open Access Journal from MDPI

Article Title: Changes in the Percentages of B- and T-Lymphocytes and Antibody Titres in Laying Hens Infested with Dermanyssus gallinae —A Preliminary Study

doi: 10.3390/ani10060987

Figure Lengend Snippet: Changes in the percentages of B IgM+ cells (Lymp B), T CD3+CD4+ cells (Lymp Th) and T CD3+CD8a+ cells (Lymp Tc) in the peripheral blood of laying hens infested with Dermanyssus gallinae , controlled with Biobeck PA 910 (AI silicon dioxide, Biologische Preparate, Brilon, Germany). (B1–B4: blood sampling dates; a,b: different letters indicate statistical differences between groups).

Article Snippet: Two microlitres of antibodies targeted at surface domains of the CD3 receptor (Mouse AntiChicken CD3-FITC clone CT-3, SouthernBiotech, Birmingham, AL, USA), CD4 receptor (Mouse AntiChicken CD4-FITC clone CT-4, SouthernBiotech, Birmingham, AL, USA) and CD8 receptor (Mouse AntiChicken CD8a-Cy5 clone 3-298, SouthernBiotech, Birmingham, AL, USA), T cells and immunoglobulin BCR (IgM + ) of B cells (Mouse AntiChickenIgM-SPRD clone M-1, SouthernBiotech, Birmingham, AL, USA) were added to each tube.

Techniques: Sampling